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c8 d30  (ATCC)


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    Structured Review

    ATCC c8 d30
    C8 D30, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c8+d30/C8-D30/us12612438-423-13-14
    Average 94 stars, based on 50 article reviews
    c8 d30 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    other:

    Article Title: Evaluation of a d -Octaarginine-linked polymer as a transfection tool for transient and stable transgene expression in human and murine cell lines
    Article Snippet: C8-D30 (mouse astrocyte), HeLa (human cervix epithelium), and RAW264.7 (mouse macrophage) cells were obtained from the American Type Culture Collection (Manassas, VA, USA).

    Cellenone:

    Article Title: Bistable type II opsins and uses thereof
    Article Snippet: Suitable cell lines include, but are not limited to, a human glioma cell line, e.g., SVGp12 (ATCC CRL-8621), CCF-STTG1 (ATCC CRL-1718), SW 1088 (ATCC HTB-12), SW 1783 (ATCC HTB-13), LLN-18 (ATCC CRL-2610), LNZTA3WT4 (ATCC CRL-11543), LNZTA3WT11 (ATCC CRL-11544), U-138 MG (ATCC HTB-16), U-87 MG (ATCC HTB-14), H4 (ATCC HTB-148), and LN-229 (ATCC CRL-2611); a human medulloblastoma-derived cell line, e.g., D342 Med (ATCC HTB-187), Daoy (ATCC HTB-186), D283 Med (ATCC HTB-185); a human tumor-derived neuronal-like cell, e.g., PFSK-1 (ATCC CRL-2060), SK-N-DZ (ATCCCRL-2149), SK-N-AS (ATCC CRL-2137), SK-N-FI (ATCC CRL-2142), IMR-32 (ATCC CCL-127), etc. .. ; a mouse neuronal cell line, e.g., BC3H1 (ATCC CRL-1443), EOC1 (ATCC CRL-2467), C8-D30 (ATCC CRL-2534), C8-S(ATCC CRL-2535), Neuro-2a (ATCC CCL-131), NB41A3 (ATCC CCL-147), SW10 (ATCC CRL-2766), NG108-15 (ATCC HB-12317); a rat neuronal cell line, e.g., PC-12 (ATCC CRL-1721), CTX TNA2 (ATCC CRL-2006), C6 (ATCC CCL-107), F98 (ATCC CRL-2397), RG2 (ATCC CRL-2433), B35 (ATCC CRL-2754), R3 (ATCC CRL-2764), SCP (ATCC CRL-1700), OA1 (ATCC CRL-6538). ..

    Article Title: Light-responsive polypeptides and methods of use thereof
    Article Snippet: Suitable cell lines include, but are not limited to, a human glioma cell line, e.g., SVGp12 (ATCC CRL-8621), CCF-STTG1 (ATCC CRL-1718), SW 1088 (ATCC HTB-12), SW 1783 (ATCC HTB-13), LLN-18 (ATCC CRL-2610), LNZTA3WT4 (ATCC CRL-11543), LNZTA3WT11 (ATCC CRL-11544), U-138 MG (ATCC HTB-16), U-87 MG (ATCC HTB-14), H4 (ATCC HTB-148), and LN-229 (ATCC CRL-2611); a human medulloblastoma-derived cell line, e.g., D342 Med (ATCC HTB-187), Daoy (ATCC HTB-186), D283 Med (ATCC HTB-185); a human tumor-derived neuronal-like cell, e.g., PFSK-1 (ATCC CRL-2060), SK-N-DZ (ATCCCRL-2149), SK-N-AS (ATCC CRL-2137), SK-N-FI (ATCC CRL-2142), IMR-32 (ATCC CCL-127), etc. .. ; a mouse neuronal cell line, e.g., BC3H1 (ATCC CRL-1443), EOC1 (ATCC CRL-2467), C8-D30 (ATCC CRL-2534), C8-S (ATCC CRL-2535), Neuro-2a (ATCC CCL-131), NB41A3 (ATCC CCL-147), SW10 (ATCC CRL-2766), NG108-15 (ATCC HB-12317); a rat neuronal cell line, e.g., PC-12 (ATCC CRL-1721), CTX TNA2 (ATCC CRL-2006), C6 (ATCC CCL-107), F98 (ATCC CRL-2397), RG2 (ATCC CRL-2433), B35 (ATCC CRL-2754), R3 (ATCC CRL-2764), SCP (ATCC CRL-1700), OA1 (ATCC CRL-6538). ..

    Article Title: Calcium indicator polypeptides and methods of use thereof
    Article Snippet: Suitable cell lines include, but are not limited to, a human glioma cell line, e.g., SVGp12 (ATCC CRL-8621), CCF-STTG1 (ATCC CRL-1718), SW 1088 (ATCC HTB-12), SW 1783 (ATCC HTB-13), LLN-18 (ATCC CRL-2610), LNZTA3WT4 (ATCC CRL-11543), LNZTA3WT11 (ATCC CRL-11544), U-138 MG (ATCC HTB-16), U-87 MG (ATCC HTB-14), H4 (ATCC HTB-148), and LN-229 (ATCC CRL-2611); a human medulloblastoma-derived cell line, e.g., D342 Med (ATCC HTB-187), Daoy (ATCC HTB-186), D283 Med (ATCC HTB-185); a human tumor-derived neuronal-like cell, e.g., PFSK-1 (ATCC CRL-2060), SK-N-DZ (ATCCCRL-2149), SK-N-AS (ATCC CRL-2137), SK-N-FI (ATCC CRL-2142), IMR-32 (ATCC CCL-127), etc. .. ; a mouse neuronal cell line, e.g., BC3H1 (ATCC CRL-1443), EOC1 (ATCC CRL-2467), C8-D30 (ATCC CRL-2534), C8-S (ATCC CRL-2535), Neuro-2a (ATCC CCL-131), NB41A3 (ATCC CCL-147), SW10 (ATCC CRL-2766), NG108-15 (ATCC HB-12317); a rat neuronal cell line, e.g., PC-12 (ATCC CRL-1721), CTX TNA2 (ATCC CRL-2006), C6 (ATCC CCL-107), F98 (ATCC CRL-2397), RG2 (ATCC CRL-2433), B35 (ATCC CRL-2754), R3 (ATCC CRL-2764), SCP (ATCC CRL-1700), OA1 (ATCC CRL-6538). ..

    Article Title: Light-responsive polypeptides and methods of use thereof
    Article Snippet: Suitable cell lines include, but are not limited to, a human glioma cell line, e.g., SVGp12 (ATCC CRL-8621), CCF-STTG1 (ATCC CRL-1718), SW 1088 (ATCC HTB-12), SW 1783 (ATCC HTB-13), LLN-18 (ATCC CRL-2610), LNZTA3WT4 (ATCC CRL-11543), LNZTA3WT11 (ATCC CRL-11544), U-138 MG (ATCC HTB-16), U-87 MG (ATCC HTB-14), H4 (ATCC HTB-148), and LN-229 (ATCC CRL-2611); a human medulloblastoma-derived cell line, e.g., D342 Med (ATCC HTB-187), Daoy (ATCC HTB-186), D283 Med (ATCC HTB-185); a human tumor-derived neuronal-like cell, e.g., PFSK-1 (ATCC CRL-2060), SK-N-DZ (ATCCCRL-2149), SK-N-AS (ATCC CRL-2137), SK-N-FI (ATCC CRL-2142), IMR-32 (ATCC CCL-127), etc. .. ; a mouse neuronal cell line, e.g., BC3H1 (ATCC CRL-1443), EOC1 (ATCC CRL-2467), C8-D30 (ATCC CRL-2534), C8-S (ATCC CRL-2535), Neuro-2a (ATCC CCL-131), NB41A3 (ATCC CCL-147), SW10 (ATCC CRL-2766), NG108-15 (ATCC HB-12317); a rat neuronal cell line, e.g., PC-12 (ATCC CRL-1721), CTX TNA2 (ATCC CRL-2006), C6 (ATCC CCL-107), F98 (ATCC CRL-2397), RG2 (ATCC CRL-2433), B35 (ATCC CRL-2754), R3 (ATCC CRL-2764), SCP (ATCC CRL-1700), OA1 (ATCC CRL-6538). .. Suitable yeast cells include, but are not limited to, Pichia pastoris, Pichia finlandica, Pichia trehalophila, Pichia koclamae, Pichia membranaefaciens, Pichia opuntiae, Pichia thermotolerans, Pichia salictaria, Pichia guercuum, Pichia pijperi, Pichia stiptis, Pichia methanolica, Pichia sp., Saccharomyces cerevisiae, Saccharomyces sp., Hansenula polymorpha, Kluyveromyces sp., Kluyveromyces lactis, Candida albicans, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Trichoderma reesei, Chrysosporium lucknowense, Fusarium sp., Fusarium gramineum, Fusarium venenatum, Neurospora crassa, Chlamydomonas reinhardtii, and the like.

    Clone Assay:

    Article Title: Oxysterol-Binding Protein ORP6 Regulates Lipid Metabolism and Brain Aβ Production.
    Article Snippet: .. Mouse astrocyte C8-D1A, C8-S, C8-D30 (astrocyte type I, II and III clones), neuroblastoma (N2A) and microglia (BV2) cell lines were obtained from the American Type Culture Collection (ATCC). .. Cells were grown and maintained in Dubelcco’s Modified Eagle Media (DMEM) (Corning, 10013CV) supplemented with 10% FBS and 1% penicillin-streptomycin (P/S) (Gibco, 15140122).

    Article Title: Oxysterol-binding protein ORP6 regulates lipid metabolism and brain Aβ production
    Article Snippet: .. Mouse astrocyte C8-D1A, C8-S, C8-D30 (astrocyte type I, II and III clones), neuroblastoma (N2A), and microglia (BV2) cell lines were obtained from the American Type Culture Collection (ATCC). .. Cells were grown and maintained in Dubelcco’s Modified Eagle Media (DMEM) (Corning, 10013CV) supplemented with 10% FBS and 1% penicillin-streptomycin (P/S) (Gibco, 15140122).

    Cell Culture:

    Article Title: Response of Astrocytes to Blood Exposure due to Shunt Insertion in vitro
    Article Snippet: .. Cell Culturing and Catheter Insertion Mouse primary astrocytes, Type III clone from the brain and cerebellum, were used for these experiments (ATCC C8-D30). .. The recommended cell culture media (ATCC) was a mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) with supplemental 50 mL of fetal bovine serum.



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    murine astrocytes  (ATCC)
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    ATCC murine astrocytes
    Figure 1. Expression of PROKR1 and PROKR2 proteins in b.End.3, <t>C8-D30,</t> and N2a cell lines. (A) Representative Western blot analysis of PROKR1 expression in b.End.3 (endothelial cells), C8-D30 <t>(astrocytes),</t> and N2a (neurons) cells. (B) Quantification of PROKR1 expression levels. (C) Represen- tative Western blot analysis of PROKR2 expression in the three cell lines, b.End.3, C8-D30, and N2a. (D) Quantification of PROKR2 expression levels. Quantification was performed with Image-J (3.53). Data are presented as mean ± SEM, from three independent experiments.
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    Image Search Results


    Figure 1. Expression of PROKR1 and PROKR2 proteins in b.End.3, C8-D30, and N2a cell lines. (A) Representative Western blot analysis of PROKR1 expression in b.End.3 (endothelial cells), C8-D30 (astrocytes), and N2a (neurons) cells. (B) Quantification of PROKR1 expression levels. (C) Represen- tative Western blot analysis of PROKR2 expression in the three cell lines, b.End.3, C8-D30, and N2a. (D) Quantification of PROKR2 expression levels. Quantification was performed with Image-J (3.53). Data are presented as mean ± SEM, from three independent experiments.

    Journal: International journal of molecular sciences

    Article Title: Effects of Prokineticins on Cerebral Cell Function and Blood-Brain Barrier Permeability.

    doi: 10.3390/ijms242015428

    Figure Lengend Snippet: Figure 1. Expression of PROKR1 and PROKR2 proteins in b.End.3, C8-D30, and N2a cell lines. (A) Representative Western blot analysis of PROKR1 expression in b.End.3 (endothelial cells), C8-D30 (astrocytes), and N2a (neurons) cells. (B) Quantification of PROKR1 expression levels. (C) Represen- tative Western blot analysis of PROKR2 expression in the three cell lines, b.End.3, C8-D30, and N2a. (D) Quantification of PROKR2 expression levels. Quantification was performed with Image-J (3.53). Data are presented as mean ± SEM, from three independent experiments.

    Article Snippet: We used murine brain microvascular endothelial cells (bEnd.3 [BEND3] (ATCC CRL-2299, LGC, Molsheim, France)), murine astrocytes (C8-D30 [Astrocyte type III clone] (ATCC CRL2534, LGC, Molsheim, France)), DMEM high glucose (#41965-039 GIBCO, ThermoFisher Scientific, Waltham, MA, USA), and N2a murine Neuroblastoma (ATCC CCL-131) DMEM Glutamax (#31966-021 GIBCO, ThermoFisher Scientific).

    Techniques: Expressing, Western Blot

    Figure 2. Effect of PROK1 and PROK2 on the proliferation of b.End3, C8-D30, and N2a cell lines. Effect of PROK1 on the proliferation of (A) endothelial cells (b.End.3), (B) astrocytes (C8-D30), and (C) neurons (N2a). Effect of PROK2 on the proliferation of (D) endothelial cells, (E) astrocytes, and (F) neurons. Data are presented as mean ± SEM, from three independent experiments; unpaired two-tailed t test was used for statistical analysis and statistical significance is indicated as * p ≤0.05, ** p ≤0.01, *** p ≤0.001, compared to the condition without prokineticin.

    Journal: International journal of molecular sciences

    Article Title: Effects of Prokineticins on Cerebral Cell Function and Blood-Brain Barrier Permeability.

    doi: 10.3390/ijms242015428

    Figure Lengend Snippet: Figure 2. Effect of PROK1 and PROK2 on the proliferation of b.End3, C8-D30, and N2a cell lines. Effect of PROK1 on the proliferation of (A) endothelial cells (b.End.3), (B) astrocytes (C8-D30), and (C) neurons (N2a). Effect of PROK2 on the proliferation of (D) endothelial cells, (E) astrocytes, and (F) neurons. Data are presented as mean ± SEM, from three independent experiments; unpaired two-tailed t test was used for statistical analysis and statistical significance is indicated as * p ≤0.05, ** p ≤0.01, *** p ≤0.001, compared to the condition without prokineticin.

    Article Snippet: We used murine brain microvascular endothelial cells (bEnd.3 [BEND3] (ATCC CRL-2299, LGC, Molsheim, France)), murine astrocytes (C8-D30 [Astrocyte type III clone] (ATCC CRL2534, LGC, Molsheim, France)), DMEM high glucose (#41965-039 GIBCO, ThermoFisher Scientific, Waltham, MA, USA), and N2a murine Neuroblastoma (ATCC CCL-131) DMEM Glutamax (#31966-021 GIBCO, ThermoFisher Scientific).

    Techniques: Two Tailed Test

    Figure 3. Effect of PROK1 and PROK2 on the migration of b.End.3, C8-D30, and N2a cell lines. Effect of PROK1 on the migration of (A) endothelial cells (b.End.3), (B) astrocytes (C8-D30), and (C) neurons (N2a). Effect of PROK2 on the migration of (D) endothelial cells, (E) astrocytes, and (F) neurons. Data are presented as mean ± SEM, from three independent experiments; unpaired two-tailed t test was used for statistical analysis and statistical significance is indicated as * p ≤0.05, ** p ≤0.01, *** p ≤0.001 compared to the condition without prokineticin.

    Journal: International journal of molecular sciences

    Article Title: Effects of Prokineticins on Cerebral Cell Function and Blood-Brain Barrier Permeability.

    doi: 10.3390/ijms242015428

    Figure Lengend Snippet: Figure 3. Effect of PROK1 and PROK2 on the migration of b.End.3, C8-D30, and N2a cell lines. Effect of PROK1 on the migration of (A) endothelial cells (b.End.3), (B) astrocytes (C8-D30), and (C) neurons (N2a). Effect of PROK2 on the migration of (D) endothelial cells, (E) astrocytes, and (F) neurons. Data are presented as mean ± SEM, from three independent experiments; unpaired two-tailed t test was used for statistical analysis and statistical significance is indicated as * p ≤0.05, ** p ≤0.01, *** p ≤0.001 compared to the condition without prokineticin.

    Article Snippet: We used murine brain microvascular endothelial cells (bEnd.3 [BEND3] (ATCC CRL-2299, LGC, Molsheim, France)), murine astrocytes (C8-D30 [Astrocyte type III clone] (ATCC CRL2534, LGC, Molsheim, France)), DMEM high glucose (#41965-039 GIBCO, ThermoFisher Scientific, Waltham, MA, USA), and N2a murine Neuroblastoma (ATCC CCL-131) DMEM Glutamax (#31966-021 GIBCO, ThermoFisher Scientific).

    Techniques: Migration, Two Tailed Test

    Figure 5. Assessment of transendothelial electrical resistance (TEER) and FD-40 passage in monolayer and co-culture models. (A) TEER measurements of endothelial cell monolayer and endothelial with astrocyte co-culture models were recorded over a 7-day culture period, with values presented in ohm.cm2. (B) The reduction of spontaneous passage of FD-40 across the endothelial monolayer and co-culture models compared to the spontaneous migration of FD-40 across the insert without cells. Data are presented as mean ± SEM, and representative of three independent experiments. Statistical analysis: two-way ANOVA corrected by Sidak test for multiple comparisons was used for TEER measurements and unpaired one-tailed t-test was used for FD-40 passage measurements. Statistical significance is indicated as * p < 0.05 and # p = 0.1.

    Journal: International journal of molecular sciences

    Article Title: Effects of Prokineticins on Cerebral Cell Function and Blood-Brain Barrier Permeability.

    doi: 10.3390/ijms242015428

    Figure Lengend Snippet: Figure 5. Assessment of transendothelial electrical resistance (TEER) and FD-40 passage in monolayer and co-culture models. (A) TEER measurements of endothelial cell monolayer and endothelial with astrocyte co-culture models were recorded over a 7-day culture period, with values presented in ohm.cm2. (B) The reduction of spontaneous passage of FD-40 across the endothelial monolayer and co-culture models compared to the spontaneous migration of FD-40 across the insert without cells. Data are presented as mean ± SEM, and representative of three independent experiments. Statistical analysis: two-way ANOVA corrected by Sidak test for multiple comparisons was used for TEER measurements and unpaired one-tailed t-test was used for FD-40 passage measurements. Statistical significance is indicated as * p < 0.05 and # p = 0.1.

    Article Snippet: We used murine brain microvascular endothelial cells (bEnd.3 [BEND3] (ATCC CRL-2299, LGC, Molsheim, France)), murine astrocytes (C8-D30 [Astrocyte type III clone] (ATCC CRL2534, LGC, Molsheim, France)), DMEM high glucose (#41965-039 GIBCO, ThermoFisher Scientific, Waltham, MA, USA), and N2a murine Neuroblastoma (ATCC CCL-131) DMEM Glutamax (#31966-021 GIBCO, ThermoFisher Scientific).

    Techniques: Co-Culture Assay, Migration, One-tailed Test